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dapi nuclear stain  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc dapi nuclear stain
    Dapi Nuclear Stain, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+nuclear+staining/DAPI/pm41818696-59-25-28
    Average 98 stars, based on 2145 article reviews
    dapi nuclear stain - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Plasma 8-OHdG act as a biomarker for steroid-induced osteonecrosis of the femoral head.
    Article Snippet: .. Then, the sections were washed three times in PBS for 5 min each and incubated with corresponding secondary antibodies at 37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for Immunoshistochemistry. .. Plasma 8-OHdG was measured using a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instruction (Cusabio, MD, USA).

    Article Title: Comparative Metabolomics Analysis Reveals Key Metabolic Mechanisms and Protein Biomarkers in Alzheimer’s Disease
    Article Snippet: .. The next day, the slices were washed three times in PBS for 5 min each and incubated with corresponding secondary antibodies at 37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for western blot. .. Statistical analysis of this study was done by SPSS, GraphPad Prism 8 (v8.0.2, https://www.graphpad.com/ ).

    Article Title: A combinatorial EVs-miRNA signature mediates the anti-tumoral activity of NFAT3-regulated extracellular vesicles in aggressive cancers
    Article Snippet: .. After blocking with PBS-Tween containing 3% BSA, cells were incubated with Alexa Fluor 488 phalloidin (Cell Signaling Technology, #8878) for 1 h, followed by DAPI nuclear staining (Cell Signaling Technology, #62248). .. Coverslips were mounted using FluoroMount-G (SouthernBiotech, #0100-01) and imaged with a ZEISS LSM800 confocal laser microscope equipped with a Plan-Apochromat 40×/1.3 NA oil immersion objective using ZEN software.

    Article Title: Plasma 8-OHdG act as a biomarker for steroid-induced osteonecrosis of the femoral head
    Article Snippet: .. Then, the sections were washed three times in PBS for 5 min each and incubated with corresponding secondary antibodies at 37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for Immunoshistochemistry. .. Plasma 8-OHdG was measured using a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instruction (Cusabio, MD, USA).

    Staining:

    Article Title: Plasma 8-OHdG act as a biomarker for steroid-induced osteonecrosis of the femoral head.
    Article Snippet: .. Then, the sections were washed three times in PBS for 5 min each and incubated with corresponding secondary antibodies at 37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for Immunoshistochemistry. .. Plasma 8-OHdG was measured using a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instruction (Cusabio, MD, USA).

    Article Title: Comparative Metabolomics Analysis Reveals Key Metabolic Mechanisms and Protein Biomarkers in Alzheimer’s Disease
    Article Snippet: .. The next day, the slices were washed three times in PBS for 5 min each and incubated with corresponding secondary antibodies at 37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for western blot. .. Statistical analysis of this study was done by SPSS, GraphPad Prism 8 (v8.0.2, https://www.graphpad.com/ ).

    Article Title: A combinatorial EVs-miRNA signature mediates the anti-tumoral activity of NFAT3-regulated extracellular vesicles in aggressive cancers
    Article Snippet: .. After blocking with PBS-Tween containing 3% BSA, cells were incubated with Alexa Fluor 488 phalloidin (Cell Signaling Technology, #8878) for 1 h, followed by DAPI nuclear staining (Cell Signaling Technology, #62248). .. Coverslips were mounted using FluoroMount-G (SouthernBiotech, #0100-01) and imaged with a ZEISS LSM800 confocal laser microscope equipped with a Plan-Apochromat 40×/1.3 NA oil immersion objective using ZEN software.

    Article Title: Plasma 8-OHdG act as a biomarker for steroid-induced osteonecrosis of the femoral head
    Article Snippet: .. Then, the sections were washed three times in PBS for 5 min each and incubated with corresponding secondary antibodies at 37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for Immunoshistochemistry. .. Plasma 8-OHdG was measured using a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instruction (Cusabio, MD, USA).

    Article Title: Activating the SDF-1/CXCR4 Axis: Notoginsenoside R1-Functionalized Zinc Scaffolds Accelerate Fracture Healing and Angiogenesis in Diabetic Osteoporosis
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Western Blot:

    Article Title: Comparative Metabolomics Analysis Reveals Key Metabolic Mechanisms and Protein Biomarkers in Alzheimer’s Disease
    Article Snippet: .. The next day, the slices were washed three times in PBS for 5 min each and incubated with corresponding secondary antibodies at 37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for western blot. .. Statistical analysis of this study was done by SPSS, GraphPad Prism 8 (v8.0.2, https://www.graphpad.com/ ).

    Blocking Assay:

    Article Title: A combinatorial EVs-miRNA signature mediates the anti-tumoral activity of NFAT3-regulated extracellular vesicles in aggressive cancers
    Article Snippet: .. After blocking with PBS-Tween containing 3% BSA, cells were incubated with Alexa Fluor 488 phalloidin (Cell Signaling Technology, #8878) for 1 h, followed by DAPI nuclear staining (Cell Signaling Technology, #62248). .. Coverslips were mounted using FluoroMount-G (SouthernBiotech, #0100-01) and imaged with a ZEISS LSM800 confocal laser microscope equipped with a Plan-Apochromat 40×/1.3 NA oil immersion objective using ZEN software.

    Microscopy:

    Article Title: Activating the SDF-1/CXCR4 Axis: Notoginsenoside R1-Functionalized Zinc Scaffolds Accelerate Fracture Healing and Angiogenesis in Diabetic Osteoporosis
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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    Image Search Results


    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

    Journal: bioRxiv

    Article Title: Lymphatic vessel dysfunction contributes to severe dengue pathogenesis

    doi: 10.64898/2026.03.27.714698

    Figure Lengend Snippet: (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

    Article Snippet: The slides were then incubated with the nuclear stain DAPI (cat# D9542, Merck) at 1:1000 dilution for 10 min and mounted onto glass slides using mounting medium (InvitrogenTM Fluoromount-GTM Mounting Medium-00495802).

    Techniques: Cell Culture, Control, Staining